Crystallization of the Bacillus subtilis histidine-containing phosphocarrier protein HPr and of some of its site-directed mutants.

doi:10.1016/0022-2836(90)90296-X

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Article

Crystallization of the Bacillus subtilis histidine-containing phosphocarrier protein HPr and of some of its site-directed mutants.

Journal of molecular biology - 5 Mar 1990

Kapadia G, Reizer J, Sutrina S L, Saier M H, Reddy P, Herzberg O

Abstract excerpt

The histidine-containing phosphocarrier protein (HPr) from Bacillus subtilis has been crystallized. Two of the site-directed mutants aimed at probing function produce crystals suitable for X-ray studies. The mutant in which His15 is substituted by an alanyl residue crystallizes from ammonium sulf...

Topics

Bacillus subtilis
Bacterial Proteins
Crystallization
Mutation
Phosphoenolpyruvate Sugar Phosphotransferase System
Polyethylene Glycols
X-Ray Diffraction

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