Article
Laboratory diagnosis and genotype identification of scrub typhus from Pinggu district, Beijing, 2008 and 2010.
The American journal of tropical medicine and hygiene - 1 Jul 2013
Li Weihong, Dou Xiangfeng, Zhang Liqin, Lyu Yanning, Du Zhizhong, Tian Lili, Zhang Xiuchun, Sun Yulan, Guan Zengzhi, Chen Lijuan, Li Xinyu, Wang Quanyi
Abstract excerpt
This study was conducted to determine the diagnosis and genotype of Orientia tsutsugamushi in Pinggu district, Beijing. Indirect immunofluorescence assay (IFA) was performed to detect O. tsutsugamushi-specific immunoglobulin M (IgM) and immunoglobulin G (IgG) antibodies. Nested polymerase chain reaction (PCR) and DNA sequencing analysis targeting the O. tsutsugamushi-specific groEL gene and 56 kDa protein gene...
Topics
- Adolescent
- Adult
- Aged
- Antibodies, Bacterial
- Base Sequence
- China
- Female
- Fluorescent Antibody Technique, Indirect
- Genotype
- Humans
- Immunoglobulin G
- Immunoglobulin M
- Male