Article
Viability and genetic stability of potato spindle tuber viroid mutants with indels in specific loops of the rod-like secondary structure.
Virus research - 15 Aug 2017
Więsyk Aneta, Candresse Thierry, Zagórski-Ostoja Włodzimierz, Góra-Sochacka Anna
Abstract excerpt
Maintenance of the rod-like structure of potato spindle tuber viroid (PSTVd), which contains over 20 loops and bulges between double-stranded helices, is important for viroid biology. To study tolerance to modifications of the stem-loop structures and PSTVd capacity for mutation repair, we have created 6 mutants carrying 3-4 nucleotides deletions or insertions at three unique restriction sites, EagI, StyI and...
Topics
- Inverted Repeat Sequences
- Solanum lycopersicum
- Mutation
- Nucleic Acid Conformation
- Plant Diseases
- RNA, Viral
- Sequence Deletion
- Solanum tuberosum
- Viroids