Article
A simple and cost-effective method to transfect small interfering RNAs into pancreatic cancer cell lines using polyethylenimine.
Pancreas - 1 Jan 2011
Wirth Matthias, Fritsche Petra, Stojanovic Natasa, Brandl Martina, Jaeckel Stephanie, Schmid Roland M, Saur Dieter, Schneider Günter
Abstract excerpt
OBJECTIVES: RNA interference, an indispensable tool in functional genomics, can be induced by small interfering RNAs (siRNAs). Because of the transient nature of siRNA-mediated RNA interference, the continuous use of transfection reagents is mandatory. Because transfection reagents are expensive,...
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