Article
Mechanistic understanding of Pyrococcus horikoshii Dph2, a [4Fe-4S] enzyme required for diphthamide biosynthesis.
Molecular bioSystems - 1 Jan 2011
Zhu Xuling, Dzikovski Boris, Su Xiaoyang, Torelli Andrew T, Zhang Yang, Ealick Steven E, Freed Jack H, Lin Hening
Abstract excerpt
Diphthamide, the target of diphtheria toxin, is a unique posttranslational modification on eukaryotic and archaeal translation elongation factor 2 (EF2). The proposed biosynthesis of diphthamide involves three steps and we have recently found that in Pyrococcus horikoshii (P. horikoshii), the first step uses an S-adenosyl-L-methionine (SAM)-dependent [4Fe-4S] enzyme, PhDph2, to catalyze the formation of a C-C...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.