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Article

Single-strand nicks induce homologous recombination with less toxicity than double-strand breaks using an AAV vector template.

Nucleic acids research - 1 Feb 2011

Metzger Michael J, McConnell-Smith Audrey, Stoddard Barry L, Miller A Dusty

Abstract excerpt

Gene targeting by homologous recombination (HR) can be induced by double-strand breaks (DSBs), however these breaks can be toxic and potentially mutagenic. We investigated the I-AniI homing endonuclease engineered to produce only nicks, and found that nicks induce HR with both plasmid and adeno-associated virus (AAV) vector templates. The rates of nick-induced HR were lower than with DSBs (24-fold lower for...

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Share to Cell Line
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Share to DNA Breaks, Double-Stranded

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 20876694
DOI: 10.1093/nar/gkq826
PMCID: PMC3035452

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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