Article
[Molecular cloning and structural-functional analysis of the arginine biosynthesis genes of the thermophilic bacterium Bacillus stearothermophilus].
Genetika - 1 Nov 1990
Sakanian V A, Ovespian A S, Mett I L, Kochikian A V, Petrosian P K
Abstract excerpt
Genes encoding arginine biosynthesis of Bacillus stearothermophilus strain 718 were cloned in the mutant argA strain of the Escherichia coli K-12. The arg genes were shown to be located on the 3.7 kb DNA fragment in the following order: argA--argE--argB. The expression of the argA gene of B. stearothermophilus on the multicopy vehicle is twofold higher in argR- strain of E. coli K-12 than is isogenic argR+...
Topics
- Arginine
- Cloning, Molecular
- DNA, Bacterial
- Escherichia coli
- Genes, Bacterial
- Geobacillus stearothermophilus
- Multigene Family
- Mutation
- Nucleic Acid Hybridization
- Restriction Mapping