Article
Establishment of a gene transfer system for Rhodococcus opacus PD630 based on electroporation and its application for recombinant biosynthesis of poly(3-hydroxyalkanoic acids).
Applied microbiology and biotechnology - 1 Oct 1999
Kalscheuer R, Arenskötter M, Steinbüchel A
Abstract excerpt
A gene transfer system for Rhodococcus opacus PD630 based on electroporation was established and optimized employing the Escherichia coli-Rhodococcus shuttle vectors pNC9501 and pNC9503 as well as the E. coli-Corynebacterium glutamicum shuttle vector pJC1 as suitable cloning vectors for R. opacus PD630, resulting in transformation efficiencies up to 1.5 x 10(5) CFUs/microgram plasmid DNA. Applying the optimized...
Topics
- Acyltransferases
- Amino Acid Sequence
- Bacterial Proteins
- Chromatography, Gas
- Culture Media
- Electrophoresis, Polyacrylamide Gel
- Electroporation
- Gene Transfer Techniques
- Genes, Bacterial
- Genetic Vectors
- Molecular Sequence Data
- Mutation
- Pseudomonas aeruginosa