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Agitation can Induce Differentiation of Human Pluripotent Stem Cells in Microcarrier Cultures

10 Aug 2010

Abstract excerpt

One of the factors that can impact human embryonic stem cell expansion in stirred microcarrier culture reactors is mechanical stress caused by agitation. Therefore, we have investigated the effects of agitation on human embryonic stem cell growth and expression of pluripotent markers. Agitation of HES-2 cell line in microcarrier cultures in stirred spinner and agitated six-well plates did not affect expression of...

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PMID: 20698747
DOI: 10.1089/ten.tec.2010.0320

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