Article
Simple and comprehensive SLA-DQB1 genotyping using genomic PCR and direct sequencing.
Tissue antigens - 1 Oct 2010
Park K, Choi H, Thong L M, Kwon O-J, Kim J-H, Lee H-T, Kim Y-B, Park S-B, Park C
Abstract excerpt
To enable the efficient analysis of a highly polymorphic swine major histocompatibility complex (MHC) class II gene, swine leukocyte antigen (SLA)-DQB1, we developed a simple and comprehensive high-resolution genotyping protocol. To obtain sufficient sequence information to design a set of common genotyping primers for SLA-DQB1, we cloned SLA-DQB1 introns 1 and 2 from 11 alleles with official four-digit allelic...
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