Article
Simple and comprehensive SLA-DQB1 genotyping using genomic PCR and direct sequencing.
Tissue antigens - 1 Oct 2010
Park K, Choi H, Thong L M, Kwon O-J, Kim J-H, Lee H-T, Kim Y-B, Park S-B, Park C
Abstract excerpt
To enable the efficient analysis of a highly polymorphic swine major histocompatibility complex (MHC) class II gene, swine leukocyte antigen (SLA)-DQB1, we developed a simple and comprehensive high-resolution genotyping protocol. To obtain sufficient sequence information to design a set of common...
Topics
- Animals
- Base Sequence
- DNA Primers
- Genetic Variation
- Genotype
- HLA-DQ Antigens
- HLA-DQ beta-Chains
- Histocompatibility Antigens Class I
- Histocompatibility Antigens Class II
- Molecular Sequence Data
- Polymerase Chain Reaction
- Reproducibility of Results
- Sequence Alignment
- Sequence Analysis, DNA
- Swine