Article
Polymerase chain reaction based genotyping for characterization of SLA-DQB and SLA-DRB alleles in domestic pigs.
Animal genetics - 1 Apr 1995
Shia Y C, Bradshaw M, Rutherford M S, Lewin H A, Schook L B
Abstract excerpt
Molecular genotyping of swine major histocompatibility complex SLA-DQB and SLA-DRB genes using polymerase chain reaction (PCR)-based amplification is described. Locus-specific oligonucleotide primers were designed for the analysis of expressed SLA genes by reverse transcription-polymerase chain r...
Topics
- Alleles
- Animals
- Base Sequence
- DNA
- DNA Primers
- Exons
- Genotype
- Histocompatibility Antigens
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- RNA-Directed DNA Polymerase
- Sequence Homology, Nucleic Acid
- Swine