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Article

Analysis of cellular phosphatidylinositol (3,4,5)-trisphosphate levels and distribution using confocal fluorescent microscopy.

Analytical biochemistry - 1 Nov 2010

Palmieri Michelle, Nowell Cameron J, Condron Melanie, Gardiner James, Holmes Andrew B, Desai Jayesh, Burgess Antony W, Catimel Bruno

Abstract excerpt

We have developed an immunocytochemistry method for the semiquantitative detection of phosphatidylinositol (3,4,5)-trisphosphate (PI(3,4,5)P3) at the cell plasma membrane. This protocol combines the use of a glutathione S-transferase-tagged pleckstrin homology (PH) domain of the general phosphoin...

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Identifiers

PMID: 20599646
DOI: 10.1016/j.ab.2010.06.033

Genes

EGF

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-20599646-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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