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Article

Biochemical characterization of two thymidylate synthases in Corynebacterium glutamicum NCHU 87078.

Biochimica et biophysica acta - 1 Sept 2010

Kan Shu-Chen, Liu Jai-Shin, Hu Hui-Yu, Chang Chia-Ming, Lin Wei-De, Wang Wen-Ching, Hsu Wen-Hwei

Abstract excerpt

The genome of Corynebacterium glutamicum NCHU 87078 contains two putative thymidylate synthase genes, designated CgthyA and CgthyX. These two genes were expressed in Escherichia coli NovaBlue and the expressed His(6)-tagged enzymes were purified by nickel-chelate chromatography. The purified CgThyA had a specific activity of 414 mU mg(-)(1) protein, whereas thymidylate synthase activity for CgThyX could not be...

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PMID: 20595007
DOI: 10.1016/j.bbapap.2010.05.006

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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