Article
Assessment of soil fungal communities using pyrosequencing.
Journal of microbiology (Seoul, Korea) - 1 Jun 2010
Lim Young Woon, Kim Byung Kwon, Kim Changmu, Jung Hack Sung, Kim Bong-Soo, Lee Jae-Hak, Chun Jongsik
Abstract excerpt
Pyrosequencing, a non-electrophoretic method of DNA sequencing, was used to investigate the extensive fungal community in soils of three islands in the Yellow Sea of Korea, between Korea and China. Pyrosequencing was carried out on amplicons derived from the 5' region of 18S rDNA. A total of 10,166 reads were obtained, with an average length of 103 bp. The maximum number of fungal phylotypes in soil predicted at...
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