Article
Barcoding lichen-forming fungi using 454 pyrosequencing is challenged by artifactual and biological sequence variation.
Genome - 1 Sept 2016
Mark Kristiina, Cornejo Carolina, Keller Christine, Flück Daniela, Scheidegger Christoph
Abstract excerpt
Although lichens (lichen-forming fungi) play an important role in the ecological integrity of many vulnerable landscapes, only a minority of lichen-forming fungi have been barcoded out of the currently accepted ∼18 000 species. Regular Sanger sequencing can be problematic when analyzing lichens since saprophytic, endophytic, and parasitic fungi live intimately admixed, resulting in low-quality sequencing reads....
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