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Article

An improved high throughput sequencing method for studying oomycete communities.

Journal of microbiological methods - 1 Mar 2015

Sapkota Rumakanta, Nicolaisen Mogens

Abstract excerpt

Culture-independent studies using next generation sequencing have revolutionized microbial ecology, however, oomycete ecology in soils is severely lagging behind. The aim of this study was to improve and validate standard techniques for using high throughput sequencing as a tool for studying oomycete communities. The well-known primer sets ITS4, ITS6 and ITS7 were used in the study in a semi-nested PCR approach...

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Identifiers

PMID: 25602160
DOI: 10.1016/j.mimet.2015.01.013

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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