Article
PMS1 from Arabidopsis thaliana: optimization of protein overexpression in Escherichia coli.
Molecular biology reports - 1 Feb 2011
Galles Celina, Gomez Rodrigo L, Spampinato Claudia P
Abstract excerpt
One of the major limitations when attempting to obtain detailed biochemical, biophysical and immunological characterization of plant DNA mismatch repair proteins is their extremely low abundance in vivo under normal growth conditions. An initial analysis of PMS1 transcript level in various Arabidopsis thaliana tissues was carried out by quantitative real-time RT-PCR. For calli, flowers and seedlings, the...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
