Article
Mutation in dipZ leads to reduced production of active human placental alkaline phosphatase in Escherichia coli.
FEMS microbiology letters - 1 Dec 1994
Beck R, Crooke H, Jarsch M, Cole J, Burtscher H
Abstract excerpt
We have tested the expression of alkaline phosphatases in a mutant strain of Escherichia coli deficient in dipZ, a gene coding for a protein involved in cytochrome c biogenesis, and the isogenic wild-type strain. The yield of soluble and active human placental alkaline phosphatase was significantly reduced in the mutant but could be fully recovered by expression of dipZ subcloned in a vector with low copy number....
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