Article
Expression analysis of desmosomal components of the novel canine epidermal keratinocyte cell line (MSCEK).
The Journal of veterinary medical science - 1 Nov 2010
Ide Kaori, Yamamoto Waka, Sato Masafumi, Yabuzoe Atsushi, Iwasaki Toshiroh, Nishifuji Koji
Abstract excerpt
A novel canine epidermal keratinocyte cell line, MSCEK, was developed from skin of a healthy dog. The aim of this study was to determine its expression of desmosomal components and to evaluate its use as a detection tool for circulating autoantibodies in canine pemphigus. Immunofluorescence and western blotting analyses revealed that MSCEK expresses desmoglein (Dsg) 1, Dsg2, Dsg3, desmoplakin, plakoglobin and...
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