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Article

A cellular screening assay using analysis of metal-modified fluorescence lifetime.

Biophysical journal - 2 Jun 2010

Cade Nicholas I, Fruhwirth Gilbert, Archibald Stephen J, Ng Tony, Richards David

Abstract excerpt

Current methods for screening cell receptor internalization often require complex image analysis with limited sensitivity. Here we describe a novel bioassay based on detection of changes in global fluorescence lifetime above a gold substrate, with superresolution axial sensitivity and no need for image analysis. We show that the lifetime of enhanced green fluorescent protein expressed in a cellular membrane is...

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Identifiers

PMID: 20513420
DOI: 10.1016/j.bpj.2010.03.016
PMCID: PMC2877351

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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