Article
Identification of the disulfide-linked homodimer of apolipoprotein E3 in plasma. Impact on receptor binding activity.
The Journal of biological chemistry - 25 Jun 1991
Weisgraber K H, Shinto L H
Abstract excerpt
The nature of disulfide-linked structures of apolipoprotein (apo) E3 in the plasma of E3/3 subjects was examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis performed under nonreducing conditions followed by immunoblotting with apoE-specific antibodies. In addition to the expected presence of the heterodimer apoE3-A-II and monomeric apoE3, a band with an apparent Mr approximately 100,000 was also...
Topics
- Apolipoproteins E
- Binding, Competitive
- Blotting, Western
- Chromatography, Gel
- Culture Techniques
- Disulfides
- Electrophoresis, Polyacrylamide Gel
- Humans
- Phenotype
- Radioligand Assay
- Receptors, LDL