Article
A 454 multiplex sequencing method for rapid and reliable genotyping of highly polymorphic genes in large-scale studies.
BMC genomics - 11 May 2010
Galan Maxime, Guivier Emmanuel, Caraux Gilles, Charbonnel Nathalie, Cosson Jean-François
Abstract excerpt
BACKGROUND: High-throughput sequencing technologies offer new perspectives for biomedical, agronomical and evolutionary research. Promising progresses now concern the application of these technologies to large-scale studies of genetic variation. Such studies require the genotyping of high numbers of samples. This is theoretically possible using 454 pyrosequencing, which generates billions of base pairs of...
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