Article
Overexpression of the recA gene decreases oral but not intraperitoneal fitness of Salmonella enterica.
Infection and immunity - 1 Jul 2010
Medina-Ruiz Laura, Campoy Susana, Latasa Cristina, Cardenas Paula, Alonso Juan Carlos, Barbé Jordi
Abstract excerpt
Transcription of the Salmonella enterica recA gene is negatively controlled by the LexA protein, the repressor of the SOS response. The introduction of a mutation (recAo6869) in the LexA binding site, in the promoter region of the S. enterica ATCC 14028 recA gene, allowed the analysis of the effect that RecA protein overproduction has on the fitness of this virulent strain. The fitness of orally but not...
Topics
- Administration, Oral
- Animals
- Caco-2 Cells
- Female
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Humans
- Ileum
- Injections, Intraperitoneal
- Mice
- Mice, Inbred BALB C
- Mutation
- Rec A Recombinases
- Reverse Transcriptase Polymerase Chain Reaction