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Highly discriminatory single-nucleotide polymorphism interrogation of Escherichia coli by use of allele-specific real-time PCR and eBURST analysis.

Applied and environmental microbiology - 1 Jul 2010

Sheludchenko Maxim S, Huygens Flavia, Hargreaves Megan H

Abstract excerpt

In total, 782 Escherichia coli strains originating from various host sources have been analyzed in this study by using a highly discriminatory single-nucleotide polymorphism (SNP) approach. A set of eight SNPs, with a discrimination value (Simpson's index of diversity [D]) of 0.96, was determined using the Minimum SNPs software, based on sequences of housekeeping genes from the E. coli multilocus sequence typing...

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PMID: 20453128
DOI: 10.1128/AEM.00128-10
PMCID: PMC2897452

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