Article
Proteomic and functional characterization of a Chlamydomonas reinhardtii mutant lacking the mitochondrial alternative oxidase 1.
Journal of proteome research - 4 Jun 2010
Mathy Grégory, Cardol Pierre, Dinant Monique, Blomme Arnaud, Gérin Stéphanie, Cloes Marie, Ghysels Bart, DePauw Edwin, Leprince Pierre, Remacle Claire, Sluse-Goffart Claudine, Franck Fabrice, Matagne René F, Sluse Francis E
Abstract excerpt
In the present work, we have isolated by RNA interference and characterized at the functional and the proteomic levels a Chlamydomonas reinhardtii strain devoid of the mitochondrial alternative oxidase 1 (AOX1). The AOX1-deficient strain displays a remarkable doubling of the cell volume and biomass without alteration of the generation time or change in total respiratory rate, with a significantly higher ROS...
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