Article
Down-regulation of cystic fibrosis gene mRNA transcript levels and induction of the cystic fibrosis chloride secretory phenotype in epithelial cells by phorbol ester.
The Journal of biological chemistry - 5 Jun 1991
Trapnell B C, Zeitlin P L, Chu C S, Yoshimura K, Nakamura H, Guggino W B, Bargon J, Banks T C, Dalemans W, Pavirani A
Abstract excerpt
To evaluate the hypothesis that phorbol myristate acetate (PMA) might modulate the expression of the cystic fibrosis (CF) gene in epithelial cells, we examined the effect of PMA on CF mRNA levels and regulation of Cl- secretion. Strikingly, PMA down-regulated CF mRNA transcript numbers in a dose- and time-dependent manner. Importantly, in parallel with the reduction of CF mRNA levels, PMA-treated cells were...
Topics
- Blotting, Northern
- Chlorides
- Colforsin
- Cystic Fibrosis
- Down-Regulation
- Epithelial Cells
- Epithelium
- Humans
- Phenotype
- RNA, Messenger
- Tetradecanoylphorbol Acetate