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Article

The human Cdc34 carboxyl terminus contains a non-covalent ubiquitin binding activity that contributes to SCF-dependent ubiquitination.

The Journal of biological chemistry - 4 Jun 2010

Choi Yun-Seok, Wu Kenneth, Jeong Kwiwan, Lee Daeyoup, Jeon Young Ho, Choi Byong-Seok, Pan Zhen-Qiang, Ryu Kyoung-Seok, Cheong Chaejoon

Abstract excerpt

Cdc34 is an E2 ubiquitin-conjugating enzyme that functions in conjunction with SCF (Skp1.Cullin 1.F-box) E3 ubiquitin ligase to catalyze covalent attachment of polyubiquitin chains to a target protein. Here we identified direct interactions between the human Cdc34 C terminus and ubiquitin using NMR chemical shift perturbation assays. The ubiquitin binding activity was mapped to two separate Cdc34 C-terminal...

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PMID: 20353940
DOI: 10.1074/jbc.M109.090621
PMCID: PMC2878539

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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