Article
Direct detection and genotyping of Toxoplasma gondii in meat samples using magnetic capture and PCR.
International journal of food microbiology - 15 May 2010
Opsteegh Marieke, Langelaar Merel, Sprong Hein, den Hartog Laurien, De Craeye Stéphane, Bokken Gertie, Ajzenberg Daniel, Kijlstra Aize, van der Giessen Joke
Abstract excerpt
Different transmission routes, including the ingestion of undercooked meat, can result in Toxoplasma gondii infection in humans. The development of effective prevention strategies is hampered by a lack of quantitative information on the contamination level of different types of meat. Therefore, we developed a method for detection and quantification of T. gondii. The method involved preparation of crude DNA...
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