[Positional cloning of a novel allele of zebrafish cloche mutant].

Abstract excerpt

OBJECTIVE: To perform the genetic identification of cloche(172) mutant zebrafish. METHODS: The chemical mutagen N-ethyl-N-nitrosourea (ENU) was used to treat the AB stain male fish. Large-scale forward genetic screening was carried out to search for lyC-deficient zebrafish mutant by WISH. The morphology changes of the embryos at 3 days postfertilization (3dpf) stage were observed and the cloche(172) gene was...

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