Comparative evaluation of an automated repetitive-sequence-based PCR instrument versus pulsed-field gel electrophoresis in the setting of a Serratia marcescens nosocomial infection outbreak.

doi:10.1128/JCM.01528-09

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Article

Comparative evaluation of an automated repetitive-sequence-based PCR instrument versus pulsed-field gel electrophoresis in the setting of a Serratia marcescens nosocomial infection outbreak.

Journal of clinical microbiology - 1 May 2010

Ligozzi Marco, Fontana Roberta, Aldegheri Marco, Scalet Giovanna, Lo Cascio Giuliana

Abstract excerpt

A semiautomated, repetitive-sequence-based PCR (rep-PCR) instrument (DiversiLab system) was evaluated in comparison with pulsed-field gel electrophoresis (PFGE) to investigate an outbreak of Serratia marcescens infections in a neonatal intensive care unit (NICU). A selection of 36 epidemiologically related and 8 epidemiologically unrelated isolates was analyzed. Among the epidemiologically related isolates, PFGE...

Topics

Adult
Automation
Bacterial Typing Techniques
Cluster Analysis
Cross Infection
DNA, Bacterial
Disease Outbreaks
Electrophoresis, Gel, Pulsed-Field
Environmental Microbiology
Genotype

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Hand

Humans

Infant, Newborn

Intensive Care Units, Neonatal

Italy

Molecular Epidemiology

Nurses

Polymerase Chain Reaction

Source

Open in PubMed Open full text

Identifiers

PMID: 20237095
DOI: 10.1128/JCM.01528-09
PMCID: PMC2863922

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-20237095-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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