Comparison of pulsed-gel electrophoresis and a commercial repetitive-element PCR method for assessment of methicillin-resistant Staphylococcus aureus clustering in different health care facilities.

doi:10.1128/JCM.03466-13

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Article

Comparison of pulsed-gel electrophoresis and a commercial repetitive-element PCR method for assessment of methicillin-resistant Staphylococcus aureus clustering in different health care facilities.

Journal of clinical microbiology - 1 Jun 2014

Crnich Christopher J, Duster Megan, Warrack Simone, Maki Dennis, Safdar Nasia

Abstract excerpt

Pulsed-field gel electrophoresis (PFGE) is a common method used to type methicillin-resistant Staphylococcus aureus (MRSA) in nosocomial investigations and epidemiological studies but is time-consuming and methodologically challenging. We compared typing results obtained using a commercial repetitive-element PCR (rep-PCR) system with PFGE in a sample of 86 unique MRSA isolates recovered from subjects in an...

Topics

Adolescent
Adult
Bacterial Typing Techniques
Cohort Studies
Cross Infection
Electrophoresis, Gel, Pulsed-Field
Environmental Microbiology
Female
Genotype
Health Facilities

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Humans

Longitudinal Studies

Male

Methicillin-Resistant Staphylococcus aureus

Molecular Epidemiology

Molecular Typing

Polymerase Chain Reaction

Staphylococcal Infections

Source

Open in PubMed Open full text

Identifiers

PMID: 24671801
DOI: 10.1128/JCM.03466-13
PMCID: PMC4042735

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-24671801-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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