Article
Comparison of automated repetitive-sequence-based polymerase chain reaction and spa typing versus pulsed-field gel electrophoresis for molecular typing of methicillin-resistant Staphylococcus aureus.
Diagnostic microbiology and infectious disease - 1 Jan 2011
Church Deirdre L, Chow Barbara L, Lloyd Tracie, Gregson Daniel B
Abstract excerpt
Automated repetitive polymerase chain reaction (PCR) (DiversiLab, bioMérieux, St. Laurent, Quebec, Canada) and single locus sequence typing of the Staphylococcus protein A (spa) gene with spa-type assignment by StaphType RIDOM software were compared to pulsed-field gel electrophoresis (PFGE) as the "gold standard" method for methicillin-resistant Staphylococcus aureus (MRSA) typing. Fifty-four MRSA isolates were...
Topics
- Cluster Analysis
- Electrophoresis, Gel, Pulsed-Field
- Genotype
- Humans
- Methicillin-Resistant Staphylococcus aureus
- Molecular Epidemiology
- Molecular Typing
- Polymerase Chain Reaction
- Repetitive Sequences, Nucleic Acid
- Staphylococcal Infections
- Time Factors