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Increased Sensitivity and Specificity of<i>Borrelia burgdorferi</i>16S Ribosomal DNA Detection

15 Mar 2010

Abstract excerpt

The DNA of Borrelia burgdorferi spirochetes extracted by ammonium hydroxide was used as the template for nested polymerase chain reaction (PCR) amplification of the species-specific 16S ribosomal DNA (rDNA). The primers were those well known to be specific for signature sequence amplification of the B burgdorferi sensu lato 16S ribosomal RNA gene. The positive 293-base-pair nested PCR amplicon was subjected to...

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PMID: 20231610
DOI: 10.1309/ajcpi72yaxrhyhee

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