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A Reverse Transcriptase–Polymerase Chain Reaction Assay of <i>Borrelia burgdorferi</i> 16S rRNA for Highly Sensitive Quantification of Pathogen Load in a Vector

1 Mar 2006

Abstract excerpt

We developed a real-time quantitative detection assay for the pathogen Borrelia burgdorferi, a Lyme borreliosis (LB) agent, using reverse transcription-polymerase chain reaction (RT-PCR) with primers and probe for a Borrelia genus-specific region of 16S ribosomal RNA. The standard curve of the assay was linear by semi-log plot over more than five orders of magnitude, and the detection limit of the assay was one...

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PMID: 16584333
DOI: 10.1089/vbz.2006.6.103

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