Article
Separation and characterization of two alpha 1,2-mannosyltransferase activities from Saccharomyces cerevisiae.
The Journal of biological chemistry - 5 May 1991
Lewis M S, Ballou C E
Abstract excerpt
Two GDP-mannose-dependent mannosyltransferase activities (designated M1MT-I and M2MT-I) from Triton X-100 extracts of Saccharomyces cerevisiae mnn1 microsomes were separated by concanavalin A lectin chromatography and partially purified. The two transferases were distinguished by differences in concanavalin A affinity and in carbohydrate acceptor specificity. Analyses of the reaction products indicate that both...
Topics
- Carbohydrate Sequence
- Chromatography, Gel
- Chromatography, High Pressure Liquid
- Guanosine Diphosphate
- Mannose
- Mannosyltransferases
- Microsomes
- Molecular Sequence Data
- Mutation
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
