Article
Cell wall glucomannoproteins of Saccharomyces cerevisiae mnn9.
Yeast (Chichester, England) - 1 Oct 1991
Van Rinsum J, Klis F M, van den Ende H
Abstract excerpt
Mannoproteins were isolated from Saccharomyces cerevisiae mnn9 mutant cell walls by laminarinase digestion and purified by affinity and anion-exchange chromatography. The purified mannoprotein fraction contained three predominant proteins with molecular masses of 300 kDa, 220 kDa and 160 kDa. These compounds were absent in an SDS extract of cell walls or in a hot-citrate extract of mnn9 cells. The carbohydrate...
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