Article
Polymerase chain reaction detection of the Dde I polymorphism in the factor 9 gene for fragile X linkage analysis.
American journal of medical genetics - 1 Jan 2000
Dobkin C S, Driscoll M C, Ferrando C
Abstract excerpt
We have used the polymerase chain reaction (PCR) to analyze the 50 base pair (bp) insertion/deletion polymorphism in the coagulation factor 9 gene. This procedure is particularly applicable for DNA marker studies in fragile X families. The polymorphism, which can also be detected in Dde I digestions, was detected by the amplification of fragments of 298 and 348 bp. The alleles were distinguished directly by...
Topics
- Alleles
- Base Sequence
- Electrophoresis, Agar Gel
- Factor IX
- Fragile X Syndrome
- Genetic Markers
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Genetic