Sequence-based analysis of secondary-metabolite biosynthesis in marine actinobacteria.

doi:10.1128/AEM.02852-09

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Article

Sequence-based analysis of secondary-metabolite biosynthesis in marine actinobacteria.

Applied and environmental microbiology - 1 Apr 2010

Gontang Erin A, Gaudêncio Susana P, Fenical William, Jensen Paul R

Abstract excerpt

A diverse collection of 60 marine-sediment-derived Actinobacteria representing 52 operational taxonomic units was screened by PCR for genes associated with secondary-metabolite biosynthesis. Three primer sets were employed to specifically target adenylation domains associated with nonribosomal peptide synthetases (NRPSs) and ketosynthase (KS) domains associated with type I modular, iterative, hybrid, and enediyne...

Topics

Actinobacteria
Cluster Analysis
DNA Primers
DNA, Bacterial
Gene Transfer, Horizontal
Genes, Bacterial
Genotype
Geologic Sediments
Molecular Sequence Data
Polymerase Chain Reaction
Sequence Analysis, DNA
Sequence Homology

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Identifiers

PMID: 20154113
DOI: 10.1128/AEM.02852-09
PMCID: PMC2849207

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