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Escherichia coli expression, refolding and characterization of human laforin.

Protein expression and purification - 1 Jun 2010

Castanheira Pedro, Moreira Susana, Gama Miguel, Faro Carlos

Abstract excerpt

Laforin is a unique human dual-specificity phosphatase as it contains an amino terminal carbohydrate binding module (CBM). Laforin gene mutations lead to Lafora disease, a progressive myoclonus epilepsy with an early fatal issue. Previous attempts to produce recombinant laforin faced various difficulties, namely the appearance of protein inclusion bodies, the contamination with bacterial proteins and a high...

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PMID: 20152902
DOI: 10.1016/j.pep.2010.02.004

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