Article
Screening for transgenic Japanese quail offspring.
Cold Spring Harbor protocols - 1 Jan 2009
Poynter Greg, Huss David, Lansford Rusty
Abstract excerpt
After mosaic founder breeding pairs of Japanese quail start to produce fertile eggs, the hatchlings must be screened for germ-line transmission to the subsequent G1 generation. This article describes how to isolate hatchling genomic DNA from the chorioallantoic membrane (CAM), which remains insid...
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