Article
Consistent production of transgenic chickens using replication-deficient retroviral vectors and high-throughput screening procedures.
Poultry science - 1 Feb 2002
Harvey A J, Speksnijder G, Baugh L R, Morris J A, Ivarie R
Abstract excerpt
We have developed a novel method of DNA extraction combined with a high-throughput method of gene detection allowing thousands of potentially transgenic chicks to be screened quickly and reliably. By using this method and a replication-deficient retroviral vector based on avian leukosis virus (ALV), we have demonstrated germline transmission of three different transgenes. Several generations of chickens carrying...
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