Article
Establishment of cell lines from individual zebrafish embryos.
Laboratory animals - 1 Oct 2023
Geyer Nathalie, Kaminsky Sabrina, Confino Shir, Livne Zohar Ben-Moshe, Gothilf Yoav, Foulkes Nicholas S, Vallone Daniela
Abstract excerpt
With the increasing use of fish as model species for research, cell cultures derived from caudal fin explants as well as pre-hatching stage embryos have provided powerful in vitro tools that can complement or serve as an ethically more acceptable alternative to live animal experiments. The widely-used protocols to establish these lines require, as a starting point, homogeneous pools of embryos or viable adult...
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