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Simultaneous Detection of <i>Salmonella</i> Strains and <i>Escherichia coli</i> O157:H7 with Fluorogenic PCR and Single-Enrichment-Broth Culture

1 Dec 2000

Abstract excerpt

A multiplex fluorogenic PCR assay for simultaneous detection of pathogenic Salmonella strains and Escherichia coli O157:H7 was developed and evaluated for use in detecting very low levels of these pathogens in meat and feces. Two sets of primers were used to amplify a junctional segment of virulence genes sipB and sipC of Salmonella and an intragenic segment of gene eae of E. coli O157:H7. Fluorogenic reporter...

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PMID: 11097930
DOI: 10.1128/aem.66.12.5472-5476.2000

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