Article
Crystallization and preliminary X-ray analysis of a monomeric mutant of Azami-Green (mAG), an Aequorea victoria green fluorescent protein-like green-emitting fluorescent protein from the stony coral Galaxea fascicularis.
Acta crystallographica. Section F, Structural biology and crystallization communications - 1 Dec 2009
Ebisawa Tatsuki, Yamamura Akihiro, Kameda Yasuhiro, Hayakawa Kou, Nagata Koji, Tanokura Masaru
Abstract excerpt
Monomeric Azami-Green (mAG) from the stony coral Galaxea fascicularis is the first monomeric green-emitting fluorescent protein that is not a derivative of Aequorea victoria green fluorescent protein (avGFP). mAG and avGFP are 27% identical in amino-acid sequence. Diffraction-quality crystals of recombinant mAG were obtained by the sitting-drop vapour-diffusion method using PEG 3350 as the precipitant. The mAG...
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