Article
Analysis of the KIR repertoire in human NK cells by flow cytometry.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2010
Björkström Niklas K, Fauriat Cyril, Bryceson Yenan T, Sandberg Johan K, Ljunggren Hans-Gustaf, Malmberg Karl-Johan
Abstract excerpt
Human natural killer (NK) cells are regulated by a diverse receptor repertoire. This makes multi-color flow cytometry-based approaches highly attractive for detailed phenotypical evaluation of NK cells. Several functional parameters can also be evaluated using this technology. In the present chapter, we demonstrate the applicability of this technology for the analysis of the human killer cell Ig-like receptor...
Topics
- Animals
- Color
- Feasibility Studies
- Flow Cytometry
- Haplotypes
- Humans
- Killer Cells, Natural
- Phenotype
- Receptors, KIR
- Staining and Labeling
