Article
Characterisation of short tandem repeats for genotyping Mycobacterium leprae.
Leprosy review - 1 Sept 2009
Gillis Thomas, Vissa Varalakshmi, Matsuoka Masanori, Young Saroj, Richardus Jan Hendrik, Truman Richard, Hall Barry, Brennan Patrick
Abstract excerpt
OBJECTIVE: Establish a typing system for Mycobacterium leprae based on polymorphic DNA structures known as short tandem repeats (STR). DESIGN: Assess 16 polymorphic STR for sensitivity, specificity and reproducibility in standard assays using reference strains of M. leprae. RESULTS: Primers for 16 STR loci were selected based on PCR product size and for their ability to sequence each STR locus from both...
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