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Article

Solution structure of the IIAChitobiose-IIBChitobiose complex of the N,N'-diacetylchitobiose branch of the Escherichia coli phosphotransferase system.

The Journal of biological chemistry - 5 Feb 2010

Jung Young-Sang, Cai Mengli, Clore G Marius

Abstract excerpt

The solution structure of the IIA-IIB complex of the N,N'-diacetylchitobiose (Chb) transporter of the Escherichia coli phosphotransferase system has been solved by NMR. The active site His-89 of IIA(Chb) was mutated to Glu to mimic the phosphorylated state and the active site Cys-10 of IIB(Chb) was substituted by serine to prevent intermolecular disulfide bond formation. Binding is weak with a K(D) of...

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Identifiers

PMID: 19959833
DOI: 10.1074/jbc.M109.080937
PMCID: PMC2823556

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