Article
Characterization of a new beta(1-3)-glucan branching activity of Aspergillus fumigatus.
The Journal of biological chemistry - 22 Jan 2010
Gastebois Amandine, Mouyna Isabelle, Simenel Catherine, Clavaud Cécile, Coddeville Bernadette, Delepierre Muriel, Latgé Jean-Paul, Fontaine Thierry
Abstract excerpt
A new HPLC method was developed to separate linear from beta(1-6)-branched beta(1-3)-glucooligosaccharides. This methodology has permitted the isolation of the first fungal beta(1-6)/beta(1-3)-glucan branching transglycosidase using a cell wall autolysate of Aspergillus fumigatus (Af). The encoding gene, AfBGT2 is an ortholog of AfBGT1, another transglycosidase of A. fumigatus previously analyzed (Mouyna, I.,...
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