Article
Purification of hamster dihydroorotate synthetase using Procion blue-Sepharose.
Protein expression and purification - 1 Sept 1990
Crofts L, Peide Y, Woodhouse A, Algar E M, Christopherson R I
Abstract excerpt
Dihydroorotate (DHO) synthetase is a trifunctional protein that catalyzes the first three reactions of de novo pyrimidine biosynthesis. A single-step procedure for purification of DHO synthetase from mutant hamster cells that overproduce this protein has been developed. The synthetase is adsorbed from a postmitochondrial supernatant to a column of Procion blue-Sepharose 4B and, after the column is washed, the...
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