Purification of hamster dihydroorotate synthetase using Procion blue-Sepharose.

doi:10.1016/1046-5928(90)90044-y

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Article

Purification of hamster dihydroorotate synthetase using Procion blue-Sepharose.

Protein expression and purification - 1 Sept 1990

Crofts L, Peide Y, Woodhouse A, Algar E M, Christopherson R I

Abstract excerpt

Dihydroorotate (DHO) synthetase is a trifunctional protein that catalyzes the first three reactions of de novo pyrimidine biosynthesis. A single-step procedure for purification of DHO synthetase from mutant hamster cells that overproduce this protein has been developed. The synthetase is adsorbed...

Topics

Animals
Aspartate Carbamoyltransferase
Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)
Cell Line
Chromatography, Agarose
Coloring Agents
Cricetinae
Dihydroorotase
Multienzyme Complexes
Mutation
Triazines

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