Article
Gene transcription analysis of carrot allergens by relative quantification with single and duplex reverse transcription real-time PCR.
Analytical and bioanalytical chemistry - 1 Jan 2010
Zagon Jutta, Jansen Bärbel, Knoppik Meike, Ehlers Anke, Kroh Lothar W, Holzhauser Thomas, Vieths Stefan, Broll Hermann
Abstract excerpt
Single and duplex real-time polymerase chain reaction (PCR) systems have been developed to quantify specific mRNA transcription of genes coding for the major Daucus carota allergen isoforms Dau c 1.01 and Dau c 1.02. Methods were tested with samples from the local market. Whereas the gene transcription levels for Dau c 1.01 were consistently high in all investigated samples, significant differences for the Dau c...
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